Ethyl chloroformate initiation system

For the sample mixmre with an unknown composition, the search for the suitable two-phase solvent system may be initiated with the partition coefficient measurement with n-hexane/ethyl acetate/n-butanol/methanol/water (5 5 0 5 5) (Table 1), chloroform/methanol/water (10 3 7) (Table 2) or ferf-butyl methyl ether/butanol/ acetonitorile/water (6 0 3 8) (Table 3). If the Ai(org/aq) value is too large, the search should be directed toward the more hydrophobic solvent systems and, if the K value is too small, the search should be directed toward the more hydrophilic solvent systems until the proper K values are obtained. 

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