Ethanolamine blocking unreacted

To block unreacted aldehyde sites, add 20 pi of 3 M ethanolamine (pH adjusted to desired value with HC1) per ml of the conjugation solution volume. React for 15 minutes at room temperature. 

Block unreacted aldehydes by the addition of 50 pi of 1M ethanolamine, pH 7.5, per ml of reaction solution. Block for 30minutes at room temperature with mixing. 

Block unreacted aldehyde sites by addition of 50 pi of 1M ethanolamine, pH 9.6, per ml of conjugation solution. Approximately a 1M ethanolamine solution may be prepared by addition of 300 pi ethanolamine to 5 ml of deionized water. Adjust the pH of the ethanolamine solution by addition of concentrated HC1, while keeping the solution cool on ice. 

Add 1 mL of 2M ethanolamine solution, and mix at 4°C for a further 1 h—this blocks unreacted active groups... 

Block unreacted aldehyde sites by addition of 50 pi of 1 M ethanolamine, pH... 

Use of membranes — Antibodies can be immobilized on membranes, which can be easily attached to the sensitive component of a transducer. Bromo-acetylcellulose membranes are first immersed in a solution containing hexamethylene diamine, and then into diepoxybutadiene, which produces the groups that will react with the antibody (Figure 3.13). Finally, the membrane with the bound antibodies is rinsed with a solution of ethanolamine to block any unreacted epoxy groups [74-76]. 

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