2- -ethanesulfonate

V-2-Hydroxyethylpiperazine-7V -2-ethanesulfonic acid (HEPES) [7365-45-9] M 238.3, pK 7.55. Crystd from hot EtOH and water. 

Sodium piperazine-A, A -bis(2-ethanesulfonate) H2O (PIPES-Na salt) [76836-02-7] M 364.3. Crystd from water and EtOH. 

N-(2-FFydroxyl)piperazine-N,-(2-ethanesulfonic acid) High performance liquid chromatography Intensity... 

HEPES (A -hydroxyethylpiperazine-A"-2-ethanesulfonic cinic acid, which has two methylene groups between its... 

Roy, R. N. Gihhons, J. J. Padron, J. L. Moeller, J., Second-stage dissociation constants of piperazine-A,A -bis(2-ethanesulfonic acid) monosodium monohydrate and related thermodynamic functions in water from 5 to 55°C, Anal. Chem. 52, 2409-2412 (1980). 

N-2-hydroxyethyl-piperazine-N -2-ethanesulfonic acid High-throughput screening... 

Fig. 15. Evolution of molecular oxygen, detected right after dissolution of 1 jjlmol of 19 into 10 ml of various solutions at various pHs at room temperature under a helium atmosphere (HEPES = A/r -2-hydroxyethylpiperazine-Af-2-ethanesulfonic acid). All the measurements were performed under a helium atmosphere in glove box equipped with a gas chromatograph. Reproduce under permission from Ref. (115). 

N-Morpholino]ethanesulfonic acid (MES) Bis[2-hydroxyethyl]iminotris(hydroxymethyl)methane (Bis-Tris) Piperazine-N,N -bis[2-ethanesulfonic acid] (PIPES)... 

N-Tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid (TES) N-2-Hydroxyethylpiperazine-N -2-ethanesulfonic acid (HEPES) 3-[N,N-Bis(2-hydroxyethyl)-amino]-2-hydroxypropane-sulfonic acid (DIPSO) 3-[N-Tris(hydroxymethyl)-methylamino-2-hydroxy-propanesulfonic acid (TAPSO) N-(2-Hydroxyethyl)piperazine-N -(2-hydroxypropanesulfonic acid) (HEPPSO)... 

Water-soluble derivatives of polythiophene have been made allowing counterions bound to the polymer backbone to self-dope with the protons (e.g., lithium and sodium ions) injecting electrons into the pi-system. Thus, combinations of sodium salts and proton salts (e.g., prepared from poly-3-(2-ethanesulfonate)thiophene) have been prepared that are both water-soluble and conducting. 

Piperazine-l,4-bis(2-ethanesulfonic acid) (PIPES) buffer Dissolve 1.385 g PIPES (Sigma) and 0.235 g sodium acetate (Merck) in 20 ml demineralized water. 

G, whereas cell-free extracts acting on penicillin N had been stimulated by ATP [19], Increasing cell mass enhanced the concentration of DAOG formed from penicillin G, the optimum concentration being 19 mg/ml. Higher cell concentrations inhibited the reaction, probably because oxygen supply was limiting under such conditions. In the studies of Cho et al. [14], the buffer used for bioconversion was 50 mM Tris-HCl [Tris = tris(hydroxymethyl)aminomethane] at pH 7.4. It was later found that 50 mM MOPS buffer or 50 mM HEPES [N-(2-hydroxyethyl) piperazine-N -(2-ethanesulfonic acid] buffer at pH 6.5 improved activity [41]. 

HEPES Ar-(2-hydroxyethyl)piperazine-A,-(2-ethanesulfonic acid) HepG2 human hepatocellular carcinoma cells HFE a gene for hemochromatosis... 

The primary fixative was a mixture of 3% glutaraldehyde, 1% formaldehyde freshly prepared from paraformaldehyde, and 0.75% tannic acid in 0.05 M piperazine-N,N -bis(2-ethanesulfonic acid) (PIPES) buffer, pH 7.0. As tannic acid may lower the pH of the fixative, it is recommended that this is checked before use and adjusted to 7.0 with 1N NaOH if necessary. The addition of low-molecular weight tannic acid to the primary fixative consistently improves the preservation of membranes and microtubules.2... 

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