Esterolytic antibody

Esterolytic antibodies have also been produced by Suga using a different bait and switch strategy (Appendix entry 2.1) (Suga et al., 1994a). A 1,2-aminoalcohol function was designed for generating not only esterases but also amidases. Of three haptens synthesized, [15], [16] and [17], two contained... 

Inspired by the great rate acceleration, by a factor of 103-104, obtained using phosphonate derivatives as TSA for the preparation of esterolytic antibodies [20],... 

Over half of the structurally characterized catalytic antibodies are from the esterolytic class of reactivity. This discussion will focus on 48G7, the most exemplary antibody from this class. In this antibody, the bound and free forms of both germline and mature antibodies have been structurally characterized, allowing for the first structural analysis of the affinity maturation process in a catalytic antibody system (Patten et al., 1996 Wedemayer et al., 1997a Wedemayer et al., 1997b). 

Crystal structures of the free and liganded form of an esterolytic catalytic antibody. J. Mol. Biol. 1997 268(2) 390-400. 60. 

Typical antibody-catalyzed reaction rates are several hundredfold to 100,000-fold faster than the uncatalyzed reaction of the substrate. Several fundamental postulates have been proposed to explain the rate enhancements that nevertheless fall short of the enormous rate accelerations of enzymes. Is activity truly due solely to transition state stabilization by antibody-binding interactions Can additional binding interactions be built into the combining site or into the substrate molecule itself to increase the overall rate of the reaction Can new screening methods and immunological methods be developed to uncover novel catalysts with diverse activities Most important, can novel esterolytic catalysts be developed based on currently available catalytic antibody technology to efficiently hydrolyze and detoxicate cocaine ... 

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