Ester adenosine 5 -pyrophosphate

The enzyme purified from bull semen dephosphorylates the 5 -phosphate esters of adenosine, uridine, cytidine, guanosine, nicotinamide riboside, and the 5 -phosphate esters of deoxynucleosides, but is inactive with adenosine pyrophosphates and substrates which bear other substituents on the ribose, e.g., adenosine 2, 5 -diphosphate (124). However 5 -nucleo-tidase from potato can hydrolyze the 5 -phosphate of this diphosphate... 

The pyrophosphorylase procedures have been applied widely for preparation of various naturally occurring a-D-glucopyranosyl esters of nucleoside pyrophosphates. For synthetic purposes, it is common to use crude or partially purified enzyme preparations, which may be a mixture of individual, specific enzymes. The synthesis of adenosine 5 -(a-D-glucopyranosyl pyrophosphate) was achieved with pyrophos-phorylases from Arthrobacter viscosus 216,217 Azotobacter vinelandii,52... 

The dependence between the reactivity of sugar nucleotides and the structure of the glycosyl group appears similar to that observed in simple glycosides, although the information available is very limited. For example, the a-D-galactopyranosyl and /J-D-glu-copyranosyl esters of adenosine 5 -pyrophosphate are cleaved more... 

Phosphorolysis of the glycosyl esters of sugar nucleotides leads to the corresponding nucleoside 5 -pyrophosphate and glycosyl phosphate. A wheat-germ enzyme specific for adenosine 5 -(a-D-glucopy-ranosyl pyrophosphate),462 and a yeast enzyme specific for guanosine 5 -(a-D-mannopyranosyl pyrophosphate),463,464 are known. 

Most of the coenzymes are esters of phosphoric or pyrophosphoric acid. The main reservoirs of biochemical energy, adenosine triphosphate (ATP), creatine phosphate, and phosphoenolpyruvate are phosphates. Many intermediary metabolites are phosphate esters, and phosphates or pyrophosphates are essential intermediates in biochemical syntheses and degradations. The genetic materials DNA and RNA are phosphodiesters. 

NAD g-Nicotinamide adenine dinucleotide Pyridinium, 3-carbamoyl-1-6-D-ribofuranosyl hydroxide, 5 - 5 - ester with adenosine 5 —(tr1 hydrogen pyrophosphate), inner salt (8) Adenosine 5 -(trihydrogen diphosphate), 5 - ... 

A synthetic method for the synthesis of 8-vinyl adenosine 5 -di and triphosphate (243a b) has been developed. This procedure eliminates unwanted depurination side-reactions under acidic conditions by introduction of acetyl protecting groups at the 2 and 3 positions of adenosine. The di- and triphosphate esters were obtained by treatment of the adenylic acid with phosphate and pyrophosphate anions.6,6-Bicyclic pyiimidopyridazin-7-one nucleoside triphosphate (244) was synthesized by nucleophilic ring-opening and rearrangement of a furanopyridine nucleoside in the presence of anhydrous hydrazine. 

Gruber and Lynen found that in the presence of triethylamine the reagent reacts with the free alcoholic group of 2, 3 -isopropylidene-adenosine (1) to give the pyrophosphate ester (2). Hydrolysis, and removal of the isopropylidine group with formic acid gave material containing 22% of adenosine diphosphate (ADP). They... 

Nucleotides are generally defined as phosphoric esters (of the carbohydrate moiety) of nucleosides. In polymerized forms (involving phospho-diester linkages), the nucleotides constitute the nucleic acids, which are fundamental components of all cells and viruses. Nucleotides also occur as mixed anhydrides, such as adenosine 5 -triphosphate and uridine 5 -(q -d-glucopyranosyl pyrophosphate), which comprise an important class of... 

Adenosine 5 -(trlhydrogen pyrophosphate), mono-D-glucopyranosyl ester 4249, 4617 ... 

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