EST clustering

As of July 2000, the human subset of UniGene contained 1.7 million sequences in 82,000 clusters 98% of these clustered sequences were ESTs, and the remaining 

000 sequences in 88,000 clusters, and rat UniGene contains 170,000 sequences in 37,000 clusters. 

Select 3 anchored clusters (contain polyadenlyation signal or tail, 

Merge some single-member clusters with other clusters (megablast) 

Chimeric, low-quality, and non-overlapping sequences rare splice forms 

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Corvin AP, Morris DW, McGhee K, Schwaiger S, Scully P, et al. 2004. Confirmation and refinement of an at-risk haplo-type for schizophrenia suggests the EST cluster, Hs.97362, as a potential susceptibility gene at the Neuregulin-1 locus. Mol Psychiatry 9 208-213. 

For ESTs the links to the known EST clusters can be given. These cross-references will also provide the links into other databases, e.g., metabolic and functional databases that describe the gene. 

In the analysis of EXAFS data on bimetallic clusters, we consider two EXAFS functions, one for each component of the clusters (8,12-15.17). If the treatment is limited to contributions of near-est neighbor backscattering atoms, each of the functions will consist of two terms. For a bimetallic cluster composed of elements a and b, the EXAFS associated with element a is given by the expression ... 

We performed a benchmark analysis on a set of genes for which the full mRNA and/or the pertinent protein sequence was documented in the literature. Instead of clustering ESTs without any template, we aligned them by a BLAST search (Altshul et al., 1997) to this set of master mRNA sequences and looked in these alignments for variant letters. We applied a set of filters as follows ... 

EST Sequence databases Bioinfofmatics Unique gene clusters... 

The 65-aa DUF 1899 exhibits a prominent cluster of highly conserved (basic) amino acids at the amino terminus representing a coronin signature that has been previously su ested to harbor a putative actin binding site just downstream of a phosphoserine modification site." The three true WD40 domains that follow may vary in some coronin isoforms due to alternative exon splicing and two sequential pseudo domains exhibit much lower detectability by sequence... 

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