ESI response factors

Hop et al. [56,59] demonstrated that ESI response factors could be normalized using nanoelectrospray. In this report, 25 compounds from 6 structurally distinct classes were tested using silicon chip-based nanoelectrospray devices (NanoMate) with <300 nL/min flow rate. The MS responses between these 25 compounds were relatively consistent (within 2.2-fold). This can be compared to standard LC-MS data which would have a 21-fold difference in response therefore, it was a big improvement. 

---