Erythrocytes adrenergic receptors

Stadel JM, Nambi P, Shorr RG, Sawyer DF, Caron MG, Lefkowitz RJ (1983) Catecholamine-induced desensitization of turkey erythrocyte adenylate cyclase is associated with phosphorylation of the beta-adrenergic receptor. Proc Natl Acad Sd USA 80( 11 ) 3173—3177... 

In cases where it has proved impractical to assay for activity at each step, protein purification has been aided by tagging a fraction of the molecules with a photoaffinity reagent (e.g. the lactose carrier of Escherichia coli Newman et al., 1981 and the /J-adrenergic receptor of frog erythrocytes Shorr et al., 1982). 

Tolkovsky AM, Levitzki A. Mode of coupling between the P-adrenergic receptor and adenylate cyclase in turkey erythrocytes. Biochemistry 1978 17 3795-3810. 

Vauquelin G, Bottari S, KanarekL, Strosberg AD. Evidence for essential disulfide bonds in Pj-adrenergic receptors of turkey erythrocyte membranes. Inactivation by dithiothreitol. J Biol Chem 1979 254 4462-4469. 

TABLE 17.2 Thermodynamic Parameters of Ligands Binding to the P-adrenergic Receptor of Turkey Erythrocytes and the Equilibrium Constants Kq at 37°... 

Table 15.1 Thermodynamic parameters of ligand binding to the p-adrenergic receptor of turkey erythrocytes and the equilibrium constants Kq at 37°C (adapted from reference 1)...

Cervantes-Oliver, P., Durieutrautman, O., Delavierklutchko, C. Strosberg, A.D. (1985). The oligosaccharide moiety of the / i-adrenergic receptor from turkey erythrocytes has a biantennary N-acetyllactosamine-containing structure. Biochemistry, 24, 3765-70. 

Luxembourg, A., Hekman, M. Ross, E.M. (1991). Turkey erythrocyte j -adrenergic-receptor-selective proteolysis of both domains. FEBS Lett., 283, 155—8. 

Rooney, T.A., Hager, R. Thomas, A.P. (1991). -Adrenergic receptor-mediated phospholipase-C activation independent of cAMP formation in turkey erythrocyte-membranes. ]. Biol. Chem., 266, 15068-74. 

Alprenolol (42), (43) Affinity chromatographic purification of the /Sj-adrenergic receptor from turkey erythrocytes 341 ... 

Limbird, L.E. and Lefkowitz, R.J. (1976) Negative cooperativity among beta-adrenergic receptors in frog erythrocyte membranes. The Journal of Biological Chemistry, 251, 5007-5014. 

Very recently a potential affinity label for the )8-adrenergic receptor in erythrocytes has been prepared by treating an amine with dibromo-acetone to form the corresponding alkylated amine containing a reactive bromoketone group. This provides a synthetic procedure that should be readily adaptable to a multitude of amines. 

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