Equilibrium techniques in saturation analysis

When Kh = K] the equilibrium reaction is defined by the simple quadratic equation (Ekins et al., 1968)  

in (i) the addition of H may increase saturation from about 

0 to 100%, in (ii) from 66 to 100%, whereas in (iii) antibody is nearly always saturated for at least 95%. These differences in saturation levels have important implications for the dose-response curves. The changing of lj l+h), due to the addition of H, has no influence on the binding capacity in the third system, but the two other methods behave as if the binding capacity increases. The / measured is then inversely proportional to the extent by which I exceeds saturation level or binding capacity. 

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