Equilibrium labeling

Fig. 6.1. Equilibrium labeling for optimal discrimination of mutant clones. At high ligand concentrations, both mutant and wild-type cells are labeled to saturation at low ligand concentrations, both exhibit background labeling. At an intermediate ligand concentration, the ratio of mutant to wild-type fluorescence is maximal.

Figure 3-34. Pulse and equilibrium labeling of a specific mRNA from E. coli...

Figure 3-35. Experimental manipulations involved in pulse and equilibrium labeling protocols.

The reaction of II with AlMe2Cl regenerates I, suggesting that the role of the electron-pair acceptor acid cocatalyst in metathesis reactions may be to facilitate carbene-metallacycle equilibrium. Labeled olefin, GDH=CRR, reacts with II affording labeled II, then labeled I by subsequent reaction with AlMejCl. Thus, the required metathesis reaction cycle is completed. 

For each acid-base equilibrium, label the stronger acid, the stronger base, the weaker acid, and the weaker base.Then predict whether the position of equilibrium lies toward the right or toward the left. 

Equilibrium labeling. Equilibrium labeling measures the accumulation of a molecule being synthesized. The experimental design is substantially different. 

Figure 13.15. Equilibrium labeling. Horizontal line represents the operon. Slanting lines are the mRNA. All slanting lines represent radioactweRNA. Between each slanting line the time is amstantf in the case discussed in the text, 3 seconds).

Figure 13.16. Chart obtained with equilibrium labeling experiment as described in the text.

Tuomi, K., Kaariainen, L., and Soderlund, H., 1975, Quantitation of Semliki Forest virus RNAs in infected cells using equilibrium labeling, Nucl. Acids Res. 2 555. 

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