Equilibrium in chromatography

In preparative chromatography, we deal with favorable adsorption isotherms, which means that the concentration in the stationary phase does not increase at the same rate as does the concentration in the mobile phase. The Langmuir adsorption isotherm originally described for adsorption of gases on plane surfaces [43] has frequently been used to describe phase equilibrium in chromatography of biomolecules [44]. 

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