EPL, Expressed protein ligation

EPL Expressed protein ligation NCL Native chemical ligation PrP Prion protein... 

Inteln-Mediated Protein Ligation (IPL)/Expressed Protein Ligation (EPL) using the IMPACT System... 

Fig. 1.4 A I ntein-mediated protein ligation (IPL) (also called expressed protein ligation EPL). B The chemical mechanism of protein cyclization by the IPL/EPL approach. HAC denotes the sequence of the active site of the intein, e.g. His-Ala-Cys. CBD stands for chitin-binding domain.

Scheme 33 Expressed protein ligation (EPL) as an example for a protein ligation method generating a native bond (Left). MIC ligation generating a non-native bond between the two coupied segments (right).

For Ras proteins, there is no chaperone protein available or known to solubilize the lipidated protein thus the refolding of, for example, lipidated N- and H-Ras is not feasible. However, lipidated K-Ras 110 can be generated via expressed protein ligation (EPL), since the polybasic C-terminal region of this protein helps to solubilize the protein even when farnesylated and facilitates the purification (Scheme 36). ... 

Key Words Expressed protein ligation (EPL) intein protein engineering protein splicing purification tag. 

Figure 6 Schematic representation of protein ligation methods applied for the introduction of a lipidated peptide on proteins and an overview of the synthesized libraries of lipidated proteins as exemplified via Ras GTPases. (A) expressed protein ligation (EPL). (B) Maleimidocaproyl (MIC).

The technique of native chemical ligation (NCL) or expressed protein ligation (EPL) (42) tremendously expanded the scope of peptide/protein synthesis. This approach also has been adopted for the construction of neoglycoproteins. For complete synthetic... 

Figure 13 Semisynthesis of homogeneous glycosylated GlyCAM-1 by expression protein ligation (EPL) combined with native chemical ligation (NCL) of recombinant protein and synthetic glycopeptide fragments.

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