Enzymology of Extremely Halophilic Archaebacteria

Effect of Salt Concentration on Structural Integrity of hMDH 

Under conditions in which substrates are in large excess with respect to enzymes, the initial rate v0 of NADH oxidation by AMDH obeys the relationship 

K and [Ea] are dependent on salt concentration Cs, we may write in general that 

The first term on the right-hand side of Eq. (2) measures the dependence of the stability of the active enzyme on salt concentration the second term expresses the dependence of the enzymatic activity (the elementary rate constants) on the salt concentration. 

Under conditions in which the enzyme is stable, Eq. (2) reduces to 

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