Temperature enzyme adaptation

Figure 7. Description of the enzyme activity and protein distribution determined in beef extracts cooked to various end-point cooking temperatures (adapted from 2).

P. F. Wintrode and F. H. Arnold, Temperature adaptation of enzymes lessons from laboratory evolution, Adv, Protein Chem. 2000, 55, 161-225. 

Kuzmina, V.V. (1985). Temperature adaptations of enzymes accounting for the membrane digestion in freshwater bony fishes (In Russian). Zhumal Obshchey Biologii 46,824-837. 

Schistocephalus solidus has its plerocercoid stage in an ectotherm (the fish Gasterosteus aculeatus) and its adult stage in an endoderm (a fish-eating bird) and it serves as an excellent model for the study of temperature adaptation in parasites. Walker Barrett (922,923) have studied the effect of temperature on (a) the activities of the mitochondrial enzyme adenosine triphosphatase (ATPase) and (b) the physical state of mitochondrial membranes in adult and larval S. solidus. 

TEMPERATURE ADAPTATION OF ENZYMES LESSONS FROM LABORATORY EVOLUTION... 

In this chapter, we will outline how evolutionary protein design methods are now being used to help uncover the molecular basis for temperature adaptation in enzymes. Before doing this, however, we will briefly review how temperature affects protein stability and enzyme activity. Then we will discuss some of the results of comparative studies of enzymes isolated from organisms adapted to different temperatures and the questions that can be addressed by laboratory evolution. 

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