Enzymes molecularly imprinted silicas

Silica particles surface-imprinted with a TSA of a-chymotrypsin were applied for the enantio-selective hydrolyzation of amides. Surprisingly, the particles showed reverse enantio-selectivity, i. e., the sol-gel imprinted with the L-isomer of the enzyme s TSA showed a higher selectivity for the D-isomer of the substrate [125]. Also Ti02 gels have been imprinted, e.g., with 4-(4-propyloxypheny-lazo)benzoic acid. QCM coated with ultrathin films of this gel were prepared by an immersion process and showed selective binding of the template [ 126]. These examples demonstrate once more the broad applicability of the concept of molecular imprinting. 

Abstract Molecular imprinting has grown considerably over the last decade with more and more applications being developed. The use of this approach for the generation of enzyme-mimics is here reviewed with a particular focus on the most recent achievements using different polymer formats such as microgels and nanogels, beads, membranes and also silica nanoparticles. 

Glad, M. Norrlow, D. Sellergren, B. Siegbahn, N. Mosbach, K. Use of silane monomers for molecular imprinting and enzyme entrapment in polysiloxane-coated porous silica. J. Chromatogr. 1985, 347, 11-23. 

M. Glad, O. Norrlow, B. Sellergren, N. Siegbahn and K. Mosbach, Use of Silane Monomers for Molecular Imprinting and Enzyme Entrapment in Polysiloxane-Coated Porous Silica, / Chromatogr., 1985, 347(1), 11-23. 

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