Enzyme wiring

Electroosmotic flow, 195 End column detection, 89 Energy barrier, 16 Enzyme electrodes, 172, 174 Enzyme immunoassays, 185 Enzyme inhibition, 181 Enzyme reconstitution, 178 Enzyme wiring, 178 Equilibrium potential, 15 Ethanol electrodes, 87, 178 Exchange current, 14... 

Mao F, Mano N, Heller A. 2003. Long tethers binding redox centers to polymer backbones enhance electron transport in enzyme wiring hydrogels. J Am Chem Soc 125 4951 -4957. 

Fig. 7.15 Oxidase enzyme wired with electron transfer relays (adapted from Heller, 1999)...

Co-extensive with the development and application of redox enzyme wired with redox pol5nner to amperometric glucose biosensors by Degani and Heller, Okamoto, Skotheim, Hale and their co-workers reported a similar approach but using different redox polymer to prevent free mediator diffusion into the bulk media [3]. 

Figure 8 is a possible redox cycle occurring in an amperometric sensor for hydrogen peroxide involving enzyme-wiring of a typical enzyme (Horseradish peroxidase, HRP) with polyaniline. HRP immobilized on the electrode surface can be oxidized by H2O2 to compound I that contains an oxyferryl centre with the iron in the ferryl state (Fclv = O), and a porphyrin 7r cation radical, followed by further direct (mediatorless) electroreduction of compound I at the electrode surface to the initial HRP state [106], The electrode is considered as an electron donor. 

The power of biofuel cells is directly related to the difference between the respective redox potentials of the electroenzymatic reactions occurring at each of the two electrodes the bioanode for glucose oxidation and the biocathode for the reduction of oxygen. The cell voltage and hence the power thus depends on the mode of enzyme wiring and therefore, the direct electron transfer is the most... 

M Elmgren, Amperometnc Biosensors Based on Enzymes Wired by Redox Polymers Acta Univ Ups, Comprehensive Summaries of Uppsala Dissertions from the Faculty of Science 471 55pp Uppsala ISBN 91-554-3175-5... 

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