Enzyme Nanolithography

Enzyme nanolithography has been introduced in 2003 and has gradually advanced since, using several SPM-based and non-SPM-based techniques. 

Enzyme Nanolithography, Fig. 1 V8 protease is immobilized to the apex of an AFM probe, to create a depression in the peptide film (Fig. 2 liom Ref. [ID... 

Enzyme Nanolithography, Fig. 2 (a) SEM image of a nanopipette, (b) Nano-channels etched in BSA by trypsin deposited with NEP... 

Enzyme Nanolithography, Fig. 3 A gel-phase supported DPPC bilayer with lines which were etched into the bilayer by (PLA2), after packing defects in the lipid bilayer were induced with the AFM tip (Fig. 2 in Ref [6])... 

Enzyme Nanolithography, Fig. 4 (a) Oligonucleotide enzyme, which digests the oligonucleotide layer (Scheme SAM. (b) Deposition of DNase I with DPN. (c) Inactive 1 from Ref [7])... 

Enzyme Nanolithography, Fig. 6 (a) Alkaline phosphatase immobilized to the AFM tip produces an insoluble product in the presence of the substrate BCIP and NBT,... 

Enzyme Nanolithography, Fig. 7 Two different metals (Au and Ag) deposited along DPN-pattemed templates of enzymes as described above (Fig. 4D from Refill])... 

Enzyme Nanolithography, Fig. 8 Poly-T nanostructures grown by TdTase activity on gold pads functionalized with a 25-mer poly-T (Fig. 2 from Ref. [12])... 

Studying the quantitative aspects of enzyme lithography. Parameters such as concentrations, time of reaction, etc., and their quantitative influence on the dimensions of the resulting features wiU allow a good control over the processes and enable the transformation of enzyme nanolithography into a true technology. 

LSTM Laser Tunneling Microscope Enzyme Nanolithography... 

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