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Enzyme linked immunospot assay

Incubation of cells in wells coated with capture antibodies [Pg.347]

Incubation with alkaline phosphatase conjugated streptavidin [Pg.347]

Addition of BCIP/NBT chromogen that turns into colored spots [Pg.347]

FIGURE 13.1 Principle of ELISPOT assay. Source Reprinted with kind permission of Springer Science + Business Media from Ref. [40], [Pg.347]

Fig. 15 Induction of cellular immunity by subcutaneous immunization with OVA-encapsulating y-PGA-Phe nanoparticles. Mice were subcutaneously immunized one time with OVA alone (10 pg), 10 pg of OVA and 100 pg of NPs (OVA-NPs), 10 pg of OVA and 100 pL of complete Freund s adjuvant (OVA + CFA), or PBS (control). Splenocytes were obtained from the immunized mice on day 10 after the immunization and stimulated with the OVA peptide. The number of IFN-y-producing cells was measured by enzyme-linked immunospot assay. SFU spot forming units... Fig. 15 Induction of cellular immunity by subcutaneous immunization with OVA-encapsulating y-PGA-Phe nanoparticles. Mice were subcutaneously immunized one time with OVA alone (10 pg), 10 pg of OVA and 100 pg of NPs (OVA-NPs), 10 pg of OVA and 100 pL of complete Freund s adjuvant (OVA + CFA), or PBS (control). Splenocytes were obtained from the immunized mice on day 10 after the immunization and stimulated with the OVA peptide. The number of IFN-y-producing cells was measured by enzyme-linked immunospot assay. SFU spot forming units...
Jakobson E, Masjedi K, Ahlborg N, et al. Cytokine production in nickel-sensitized individuals analysed with enzyme-linked immunospot assay Possible implication for diagnosis. Br J Dermatol. 2002 147 442-449. [Pg.132]

Czerkinsky, C.C., and Svennerholm, A.M. (1983) Ganglioside GM1 enzyme linked immunospot assay for simple identification of heat labile enterotoxin producing... [Pg.368]

Okamoto, Y., Abe, T., Niwa, T., Mizuhashi, S.,and Nishida,M. (1998) Developmentof a dual color enzyme linked immunospot assay for simultaneous detection of murine T helper type 1 and T helper type 2 cells. Immunopharmacology, 39, 107 116. [Pg.370]

The ELISPOT (enzyme-linked immunospot) assay is based on classical immunoassay principles and the detection procedures employed in ELISA assays. Its sensitivity, ease of use, employment of highly reactive, standardized monoclonal antibodies, conunercial availability, and ready application to studies on individual cell types makes it a good choice for research and diagnostic... [Pg.122]

Czerkinsky, C.C., Nilsson,L.A., Nygren,H., Ouchterlony, O., and Tarkowski, A. (1983) A solid phase enzyme linked immunospot (ELISPOT) assay for enumeration of specific antibody secreting cells. Journal of Immunological Methods, 65, 109 121. [Pg.368]

Okamoto, Y., Murakami, H., and Nishida, M. (1997) Detection of interleukin 6 producing cells among various organs in normal mice with an improved enzyme linked immunospot (ELISPOT) assay. Endocrine Journal, 44, 349 355. [Pg.369]

See other pages where Enzyme linked immunospot assay is mentioned: [Pg.466]    [Pg.87]    [Pg.346]    [Pg.347]    [Pg.369]    [Pg.466]    [Pg.87]    [Pg.346]    [Pg.347]    [Pg.369]    [Pg.343]    [Pg.722]   
See also in sourсe #XX -- [ Pg.87 , Pg.100 ]



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