Enzyme Kinetics , xiii

TABLE XIII Enzyme Kinetics of Enterobacter cloacae B-Lactamase°... 

CAs are ubiquitous metalloenzymes that catalyze the reversible synthesis of bicarbonate ion and proton from carbon dioxide and water. This family includes 15 isoenzymes (1-XlV, V further subclassified as VA and VB) endowed with different molecular features, cellular localization, distribution in organs and tissues, and kinetic properties. It has been demonstrated that the dysfunction of these enzymes is related to different pathologies such as cancer (CAs Vll, IX, XII), neurodegeneration (CA VIII), obesity (CAs VA and VB), and sterility (CA XIII) [36]. Based on the knowledge that coumarin derivatives are CA inhibitors [37], a library of suflbcoumarin derivatives 21 bearing a triazole moiety was synthesized (Scheme 2.3). These compounds 21 (R = Ph) were found to... 

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