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Enzyme kinetics ELISA

Enzyme kinetics, 10 254-256 Enzyme-linked immunosorbent assay (ELISA), 3 801-802 14 136 Enzyme manufacturers associations,... [Pg.322]

Figure 4 deals with some of the enzymatic systems in the ELISA, and illustrates areas that need to be understood in order to allow optimal performance to be maintained. Understanding enzyme kinetics, catalysis reactions, hazards, and buffer formulation (pH control) are all essential. [Pg.2]

Chromogenic enzyme kinetic assay/photometry ELISA... [Pg.180]

ELISA assays may be competitive or noncompetitive. As the name imphes, in a competitive ELISA, enzyme-labeled antigen competes with free antigen (the analyte of interest) for a fixed and limited quantity of immobihzed antibody binding sites. After incubation, the microtiter plate (sohd support) is rinsed to remove all unbound species and the enzyme substrate is added in saturating concentration. The conversion of substrate to produce can be measured continuously (kinetic assay) or, more commonly. [Pg.211]

See other pages where Enzyme kinetics ELISA is mentioned: [Pg.488]    [Pg.168]    [Pg.87]    [Pg.48]    [Pg.83]    [Pg.408]    [Pg.113]    [Pg.203]    [Pg.131]    [Pg.83]    [Pg.74]    [Pg.74]    [Pg.186]    [Pg.1432]    [Pg.2155]    [Pg.146]    [Pg.361]    [Pg.58]    [Pg.68]    [Pg.202]    [Pg.114]   
See also in sourсe #XX -- [ Pg.8 , Pg.87 ]



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