Enzyme electrophoresis

Andrews, R.H. and Chilton, N.B. (1999) Multilocus enzyme electrophoresis a valuable technique for providing answers to problems in parasite systematics. InternationalJournal for Parasitology 29, 213—253. 

At a few British Columbia localities intermediates occur (cells isodiametric, single or in pairs, and these are intermediate between the two morphotypes (Figure 5), leading to the enzyme electrophoresis study described above. 

Harrison, L. J. S., Le Riche, P. D. Sewell, M. M. H. (1986). Variations in Echinococcus granulosus of bovine origin identified by enzyme electrophoresis. Tropical Animal Health and Production, 18 48-50. 

Identification of Echinococcus strains by enzyme electrophoresis. Research in Veterinary Science, 25 247-8. 

Geographical divergence in Bothriocephalus (Cestoda) of fishes demonstrated by enzyme electrophoresis. International Journal for Parasitology, 16 553-8. 

H. Harris and D. A. Hopkinson, Handbook of Enzyme Electrophoresis in Human Genetics. North-Holland Publ., Oxford, 1976. 

Each reaction mi. ture contained 4.6% ovalbumin and 0.01% enzyme. Electrophoresis was carried out in sodium phosphate buffer of pH 6.8 and 0.1 T/2 for 12,600 seconds at 6.2 volts per centimeter. 

Enzyme Standards. Enzyme electrophoresis has proliferated with Increased genetic profiling for medical and forensic use. Standards for enzyme analyses In the form of defined kits containing banks of enzymes of different Isoenzyme patterns are not commercially available. For the most part, forensic laboratories use Individuals from within their own laboratories who have known phenotypes for the enzymes of Interest. Such donors become de facto standards. The Interlaboratory exchange of samples and rigorous continued training In enzyme phenotype Identification will Improve this standards base. 

Selander, R.K., Caugant, D.A., Ochman, H., Musser, J.M., Gilmour, M.N., and Whittam, T.S. 1986, Methods of multilocus enzyme electrophoresis for bacterial population genetics and systematics. Applied Environmental Microbiology 51 873-884. 

MEE Multilocus enzyme electrophoresis (MEE) can be used to estimate genetic relatedness among strains or species by measuring the relative mobility of cellular enzymes under nondenaturing conditions. The allelic profiles or MEE types may be used to compare... 

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