Enzyme-albumin conjugates

M. 1. Poznansky. Targeting enzyme albumin conjugates examining the magic bullet. N.Y. Aaad, ScL 507 211 (19B7). 

M. J. Poznansky. Soluble enzyme-albumin conjugates new possibilities for enzyme replacement therapy. Methods Enzymol. 737 566-574 (1988). 

Enzyme-Albumin Conjugates Advantages and Problems with Targeting... 

Table 4 Tissue Deposition of Intravenously Injected Enzymes and Enzyme/Albumin Conjugates...

Instead of using human serum albumin as a carrier protein, other workers (135) utilized ovalbumin for preparing the diazotized clenbuterol antigen in an enzyme immunoassay developed for screening of clenbuterol residues in bovine urine, liver, and eye. Alkaline phosphatase rather than -galactosidase was also used as an enzyme label in the preparation of the enzyme-clenbuterol conjugate. 

Table 3 Plasms Clearance of Albumin-Conjugated Enzymes...

One limitation of enzyme replacement therapy is the targeting of enzyme proteins to appropriate sites of substrate accumulation. Administration of a cholesterol esterase conjugated to albumin results in the degradation of pathologic cholesterol ester accumulations within the lysosomes of fibroblasts from a patient with cholesterol ester storage disease (246). 

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