Enzyme adiabatic process

The non-adiabatic long-range electron transfer (LRET) has been proven to be one of the key stages of many processes in enzymes, proteins and model systems. Therefore, theoretical calculation and experimental determination of the resonance integral (V) and its dependence on the distance between donor and acceptor centers appears to be a fundamental problem. 

Assuming that some reaction processes are fast, they can be adiabatically eliminated. Also, most fast reversible reactions can be eliminated by assuming that they are already balanced. Then we need to discuss only the concentration (number) of molecular species, which changes relatively slowly. For example, by assuming that an enzyme is synthesized and decomposed fast, the concentrations can be eliminated to give catalytic reaction network dynamics consisting of the reactions with... 

Especially useful for enzymatic reactions, the generation of heat (enthalpy change) can be used easily and generally. The enzyme provides the selectivity and the reaction enthalpy cannot be confused with other reactions from species in a typical biologic mixture. The ideal aim is to measure total evolved heat, that is, to perform a calorimetric measurement. In real systems there is always heat loss, that is, heat is conducted away by the sample and sample container so that the process cannot be adiabatic as required for a total heat evolution measurement. As a result, temperature difference before and after evolution is measured most often. It has to be assumed that the heat capacity of the specimen and container is constant over the small temperature range usually measured. 

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