Enzyme active sites, nonpolar activation barrier

This calculation demonstrates that a nonpolar solvent can accelerate S 2 reactions. However, this is not what we are asking the relevant quantity is the overall activation energy for the reaction in a nonpolar enzyme which is surrounded by water. Thus, as is indicated in the thermodynamic cycle of Fig. 9.3, we should include the energy of moving the ionized R-O- from water to the nonpolar active site (AAg j1). Thus the actual apparent change in activation barrier is... 

---