Enzymatic surface-initiated monolayers

The first surface-initiated enzymatic polymerization reported was the synthesis of amylose brushes on planar and spherical surfaces [145]. For this, silica or silicone surfaces were modified with self-assembled monolayers of (3 amino-propyl)trimethoxysilane or chlorodimethylsilane, respectively. To these functionalities, oligosaccharides were added via (a) reductive amidation of the oligosaccharides to surface-bound amines, (b) conversion of the oiigosaccharide to the according aldonic acid lactone and reaction with surface bound amines, and (c) incorporation... 

A surface-initiated enzymatic ROP has also been reported, whereby CL and DXO were grafted from hydroxyl-terminated self-assembled monolayers (SAMs) on gold, using Novozym 435 [117], while polycaprolactone-modified hydroxyethylcel-lulose films were prepared by the enzymatic ROP of CL [118]. 

In addition, it was desired to explore the effects that these small changes in intermolecular distance had on the enzymatic susceptibility of these lecithins to hydrolytic enzymes such as phospholipase A [3-5], a potent hydrolytic enzyme found in cobra venom. Thus, microgram quantities of enzyme were injected under this monolayer. By measming the rate of change of surface potential, one can indirectly measine the rate of reaction in the monolayer. It is assumed that these quantities [i.e., change in surface potential A(AV) and the extent of reaction] are proportional to each other. The kinetics of hydrolysis, as measured by a decrease in sinface potential, were studied for each lecithin monolayer as a fimction of initial surface pressure and are shown in Fig. 3 [6]. It was foimd that initially the reaction rate... 

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