Enzymatic Quantitation of Total Starch in Plant Products

The Megazyme procedure requires the company s Total Starch Assay Kit, which contains most of the necessary enzymes and chemicals. This procedure was approved by the American Association of Cereal Chemists (AACC Method 76-13 AACC, 1976) and the Association of Official Analytical Chemists (AOAC Method 996.11) following an extensive collaborative AACC/AOAC interlaboratory evaluation (McCleary et al., 1997b). The procedure is simpler than a previously reported method (AACC, Method 76-12). The procedure by Holm et al. (1986) described in the Alternate Protocol uses enzymes and chemicals purchased individually. 

MEGAZYME TOTAL STARCH ASSAY (AMYLOGLUCOSIDASE/a-AMYLASE METHOD) 

The kit includes necessary enzymes (thermostable a-amylase and amyloglucosidase), some reagents (buffer concentrate and glucose oxidase/peroxidase [GOPOD] reagent) and standards (glucose solution and com starch) to carry out 100 starch determinations. The other reagents required for this procedure may be obtained from any chemical supplier. 

Total Starch Assay Kit (Megazyme International Ireland), containing  

Glucose oxidase/peroxidase (GOPOD) reagent (see recipe for dilution) 

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E2.1 Overview of Laboratory Isolation of Starch from Plant Materials E2.2 Enzymatic Quantitation of Total Starch in Plant Products E2.3 Determination of Total Amy lose Content of Starch... 

E2.2 Enzymatic Quantitation of Total Starch in Plant Products E2.2.1... 

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