Enzymatic PEGylation

C. Maullu, D. Raimondo, F. Caboi, A. Giorgetti, M. Sergi, M. Valentin , G. Tonon, A. Tramontano, Site-directed enzymatic PEGylation of the human granulocyte colony-stimulating factor. FEES /., 276 (22) 6741-6750, 2009. 

Fig. 5 Enzymatic site-specific PEGylation by transglutaminase (TGase). The alkylamine derivative of PEG can be introduced into proteins in a site-specific manner...

Sato H (2002) Enzymatic procedure for site-specific pegylation of proteins. Adv Drug Deliv Rev 54 487-504... 

Sato, H. "Enzymatic procedure for site-specific pegylation of proteins". Adv. Drug Deliv. Rev. 54(4), 487-504 (2002). 

M. Elsabahy, M. Zhang, S. Gan, K. C. Waldron and J. C. Leroux, Synthesis and enzymatic stability of PEGylated oligonucleotide duplexes and their self-assemblies with polyamidoamine den-drimers. Soft Matter, 4,294-302 (2008). 

L. Cui, C. Tang, and C. Yin, Effects of quaternization and PEGylation on the biocompatibility, enzymatic degradability and antioxidant activity of chitosan derivatives. Carbohydrate Polymers, 87 (4), 2505-2511,2012. 

Fig. 15 A Polymer-enzyme liposome therapy (PELT) relies on the liberation of drug from liposomes by the action of a polymer-phospholipase conjugate. B PEGylated liposomal doxorubicin (Doxil) activated by HPMA copolymer-Gly-Gly-phospholipase C conjugate. C Comparison of enzymatic activity of free phospholipase C and HPMA copolymer Gly-Gly-phospholipase C in vitro. Release of doxorubicin from Daunoxomes in the presence of phospholipase C ( ), HPMA copolymer-Gly-Gly-phospholipase C (A) or Triton X-100 (1%) ( ) and in the absence of enzyme as a control ( ) (R. Satchi and R. Duncan, unpublished results)...

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