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Enzymatic Glycosylations with Glycosyltransferases

This chapter will describe several Leloir-type transferase reactions that have been used for stepwise construction of a oligosaccharides comprising five different monosaccharides, GlcNAc, GalNAc, Gal, Fuc, and Neu5Ac. This is a very limited approach among the myriads of possible oligosaccharides that can be potentially [Pg.647]

Many plasma membrane proteins contain domains of mucin type with clustered O-linked glycans the peptide cores within these clusters adopt stiff and extended conformations, representing rods from which the saccharides protrude [5]. [Pg.648]

Since GPEs can use a variety of mechanisms to promote enzymatic glycosylation, the timing of proton transfer could in principle be different. This of course is at least partly due to the wide variety of substrates that GPEs utilize. Current modeling of glycosyltransferase mechanisms by QM-MM methods finds late TSs with relatively... [Pg.130]

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Enzymatic glycosylation

Enzymatic glycosylations

Glycosyltransferase

Glycosyltransferases

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