Enzymatic Acylation of Rutin and Silybin with Dicarboxylic Acids

1 Enzymatic Acylation of Rutin and Silybin with Dicarboxylic Acids 

Higher conversion yields were observed for all dicarboxylic acids used in the case of silybin, as compared to rutin, due to the presence of primary hydroxyls in this flavonolignan for which the lipase shows higher specificity. Analysis of the chemical structures of purified products by NMR revealed that the acylation of rutin occurred at the 4 -OH of the rhamnose moiety, while the aylation of silybin occurred at its unique primary hydroxyl at position C23. It must be noted that, in the case of silybin, both monoester and diester formation was observed and identi- 

---