Energy diagrams enzyme catalysis, 116

Fig. 2.35. Fungal a-amylase. Amylose hydrolysis versus temperature. Arrhenius diagram for assessing the activation energy of enzyme catalysis and enzyme inactivation V = total reaction rate...

Figure 1.6 Schematic representation of the changes in protein conformational microstate distribution that attend ligand (i.e., substrate, transition state, product and inhibitor) binding during enzyme catalysis. For each step of the reaction cycle, the distribution of conformational microstates is represented as a potential energy (PE) diagram.

If energy diagrams are used to answer questions about enzymic catalysis sometimes it is necessary to speeify the standard states chosen or the working concentrations and sometimes it is not. There are two cases commonly used, one is the comparison of enzyme-catalysed with non-enzyme-catalysed reactions and the other is a series of enzyme-catalysed reactions in which the enzyme or substrate is assumed to be modified in a particular manner [10,13,19,20],... 

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