0-Endorphin porcine

Ling N. Solid-phase synthesis of porcine a-endorphin and y-endorphin, two hypothalamic-pituitary peptides with opiate activity. Biochem. Biophys. Res. Commun. 1977 74 248-255. 

Kakidani H, Furutani Y, Takahashi H, Noda M, Morimoto Y, Hirose T, Asai M, Inayama S, Nakanishi S, Numa S. Cloning and sequence analysis of cDNA for porcine beta-neo-endorphin/ Dynorphin precursor. Nature 1982 298 245-249. 

P-endorphin is an endogenous 31 amino acid residue peptide present in human, porcine, ovine and bovine pituitaries, with amino acid sequences identical to the 61-91 portion of their respective P-lipotropins, It is a potent (5 and p) OPIOID RECEPTOR AGONIST and OPIOID ANALGESIC. See also endorphins. 

Richter, W.O. Schwandt, P. Separation of neuropeptides by HPLC evaluation of different supports, with anal3dical and preparative applications to human and porcine neurophysins, p-Upotropin, adrenocorticotropic hormone, and p-endorphin. J.Neu,rochem., 1985, 44, 1697—1703... 

Neoendorphins, opioid peptides derived from the precursor protein pro-dynorphin (pro-enkephalin B). a-Neoendorphin, H-Tyr-Gly-Gly-Phe-Leu-Arg-Lys-Tyr-Pro-Lys -OH, corresponds to the partial sequence of human and porcine pro-dynorphin-(175-184), and contains the sequence of -neoendorphin ([desLys ]-a-endorphin). Both neoendorphins show potent activity in the guinea pig ileum assay [K. Kangawa et al., Biochem. Biophys. Res. Commun. 1981, 99, 871 N. Minamino et al., Biochem. Biophys. Res. Commun. 1981, 99, 864]. 

Figure 16 Plots of In kj versus for several polypeptide hormones. The experimental conditions used in this study were column, Bondapak C,q (4.0 x 300 mm, dpIO m) flow rate 2.0mL/min mobile phase, 15mM triethylamine-4mM H2S04-15mM H3PO4, with the volume fraction, of acetonitrile adjusted from 0.0 to 0.8. The pH value for the 100% water experiment was pH 2.2. The key to the polypeptide structures is 1, human /J-Leu endorphin 2, human -Met -endorphin 3 porcine ACTH[l-39] 4, angiotensin I 5, oxytocin 6, angiotensin II 7, angiotensin III. (Data adapted from Refs. 162 and 580.)...

Kakidani, H., Furutani, Y., Takahashi, H., Noda, M., Morimoto, Y., Hirose, T., Asai, M., Inayama, S., Nakanishi, S., and Numa, S., 1982, Cloning and sequence analysis of cDNA for porcine p-neo-endorphin/dynorphin precursor. Nature 298 245. 

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