Encephalomyelitis, allergic/autoimmune experimental immune cells

Figure 7. The effect of K252a on the therapeutics and macrophage activation in the experimental autoimmune allergic encephalomyelitis. (A) EAE was induced by a common protocol, neurological examinations were daily performed and the mean neurological score was measured. The control animal groups received either carrier or immunized without any other treatment. (B) Peritoneal macrophages collected from the animal groups as presented in figure 7A, were isolated in F12 medium, divided in samples of 10 cells/ml and incubated with EPS (bacterial lipopolysacharides, 10 ng/ml) for 18 hours at 37°C. Thereafter NO release in the culture medium was measured as described by Denis, 1991. (C) NR-8383 rat alveolar macrophages were incubated for 18 hours at 37°C (10 cells/ml) with 1 ng/ml EPS in the presence of 1 pM mentioned alkaloids. NO release to the culture medium was measured as previously described.

---