Embedded media

SERS Substrates Based on Metal Nanoparticle-Embedded Media... 

The directions for thorough mixing of the reagents in disposable plastic beakers are included with commercially available resin kits. Most embedding media are toxic, requiring the use of chemical hoods. In addition, certain reagents can cause dermatitis and thus require the use of gloves. 

Infiltrate with a resin (see Table 4 for alternative embedding media for other than pollen tubes). 

Embed in Vestopal W. More contemporary embedding procedures would include Spurr s or Epon embedding media. The protocols for both Spurr s and Polybed (a recent em formulation) use can be found in a variety of EM books (see Chapter 1). In addition, these protocols accompany the commercially available kits. 

If samples have to be sectioned, for transmission experiments the type of embedding medium is important. Epoxy embedding media traditionally used in soil or sediment investigations (Tippkotter and Ritz, 1996) contain carbon and nitrogen... 

Preparation of Tissues Tissues are quick-frozen in OCT embedding media, and 4 to 6 pm cryosections are placed on slides and allowed to dry, or stored at 4 °C for a few days or at -80 °C for longer terms prior to fixation. Prolonged retention of tissue sections is usually to be avoided even at -80 °C as antigen deterioration can occur even under these conditions. Blood smears are prepared fresh and allowed to dry prior to fixation.Tissue preparation (sectioning, drying, fixation) is governed by the requirements needed to preserve the... 

The most common way to obtain IR spectra for quantiative evaluation is to embed the sample in an alkali halide pellet. Dry, spectroscopy-grade potassium bromide or potassium chloride are suitable embedding media. (Drying procedure 120°C in oven for at least 1 h, followed by cooling in a desiccator over... 

After the extraction, the chips are embedded in a suitable resin using a capsule or a rubber bed. Embedding media commonly used for electron microscopy are also suitable for UV microscopy. However, the well-known Epon 812 formulation of Luft (1961) shows increasing absorbance at lower wavelengths (Wood and Goring 1973, Boutelje and Jonsson 1980). Instead, the following formulation is recommended for low absorbance ... 

Plastic embedding media have been used for sections less than 1 xm thick, e.g., for electron microscopy (Falser et al., 1986 Binder et al., 1986) for cases where superior morphological detail and resolution are required. 

Lowicryl. [Lowi GmbH] Embedding media for electron microscopy. 

G. N. Thomopoulos, B. A. Schulte, and S. S. Spicer, The infiuence of embedding media and fixation on the post-embedment ultrastructural demonstration of complex carbohydrates. I. Morphology and periodic acid—thiocarbohydrazide-silver proteinate staining of vicinal diols, Histochem. J., 15 (1983) 763-784. 

Polysciences Inc., 1986. Spurr Low-viscosity Embedding Media. Polysciences data sheet 127. 

Tobler M, Wiithrich B, Freiburghaus AU (1990) Contact Dermatitis from acrylate and methacrylate compounds in Lowicryl embedding media for electron microscopy. Contact Dermatitis 23 90-102... 

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