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Eluent gradient adsorption chromatography

Eluent gradient adsorption chromatography is one of the main HPLC techniques used in polymer fractionation including deposition-dissolution, chromatography under critical conditions, temperature gradient adsorption LC and precipitation dissolution LC [24-30]. [Pg.7]

The setnp for polymer HPLC is qnite similar to SEC systems with a few modifications. Liqnid Adsorption Chromatography (LAC) requires the adsorption and desorption on a stationary phase. Therefore, in most cases isocratic separation is not sufficient. Gradients with respect to pH valne, ionic strength, eluent composition, or temperature are applied. The most common approach is to use eluent composition gradients. In contrast to SEC where polymeric phases dominate, silica-based column packings are the most important stationary phase. Both normal phase and reversed phase separations have been described. A summary of different applications in copolymer separation is offered by Pasch [27]. Detectors used in gradient LAC are mainly UV/DAD detectors and ELSD. [Pg.190]

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See also in sourсe #XX -- [ Pg.7 ]



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