Eluant fumaric acid

Figure 8 Separation of isomeric acids (maleic and fumaric acid) by controlled surface porosity anion exchange chromatography. Column Sulfonated fluoropolymer coated onto a 50-p glass bead. Average pore size about 1000 A. Flow rate 2.73 ml/min. Eluant 10 mM HN03. Temperature 60°C. Detection absorbance. (Reproduced from Kirkland, J. J., J. Chromatogr. Sci., 7,361,1969. By permission of Preston Publications, A Division of Preston Industries, Inc.)...

Fig. 3-126. Separation of maleic acid and fumaric acid. -Separator column lonPac AS4A (-SC) eluant 1.7 mmol/L NaHCOs + 1.8 mmol/L Na2C03 flow rate 2.0 mL/min detection UV (210 nm) injection 50 pL solute concentrations 2 mg/L maleic acid (1) and 5 mg/L fumaric acid (2).

Fig. 9-136. Ion-exclusion chromatographic separation of lactic acid, acetic acid, and fumaric acid in a pear nectar prior to and after incubation with Rhizopus stolonifer. - Separator column lonPac ICE-AS6 eluant 0.5 mmol/L perfluorobutyric acid flow rate 1 mL/min detection (a) suppressed conductivity, (b) UV (207 nm) sample pear nectar diluted 1 10 with deionized water, incubated sample diluted 1 1000 peaks (1) lactic acid,...

Fig. 9-209. Separation of organic acids in human serum. - Separator column CarboPac PA-100 eluant NaCi - Tris (5 mmol/L, pH 7.5) gradient 0.03 mol/L isocratic for 1 min, then linearly to 0.3 mol/L in 15 min, then rinsing step with 1 mol/L for 4 min flow rate 1 mL/min detection UV (214 nm) injection volume 30 pL sample preparation see text peaks (1) lactate, (2) acetate, (3) pyruvate, (4) malonate, (5) nitrite, (6) fumarate, (7) citrate, and (8) nitrate (taken from [339]).

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