Elongator complex stability

Kuznedelov, K., Korzheva, N., Mustaev, A., and Severinov, K. (2002). Structure-based analysis of RNA polymerase function the largest subunits rudder contributes critically to elongation complex stability and is not involved in the maintenance of RNA-DNA hybrid length. EMBO J. 21(6), 1369-1378. 

Interaction of the hybrid with its binding site ensures that the stability of the elongation complex, and thus processive transcription, is coupled with the presence of RNA. 

Kireeva, M. L., Komissarova, N., Waugh, D. S., and Kashlev, M. (2000). The 8-nucleo-tide-long RNA DNA hybrid is a primary stability determinant of the RNA polymerase II elongation complex. / Biol. Chem. 275(9), 6530-6536. 

Derivatives of aliphatic alkynes (14 and 15) are more thermally unstable than 12, but they show SmA and N phases at low temperatures (below 130 °C). The type of phase and the mesophase stability depend on the length of both the terminal and the lateral chains. When both chains are elongated, the mesomorphism becomes metastable and compounds 14 display monotropic N and SmA transitions. Complexes IS, which contains an ester group with an opposite direction to that of complexes 14, display less stable nematic mesophases. 

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