Electroporation protein expression transformation

The calli used to generate cell lines can be transgenic and express the vaccine or therapeutic protein of interest. Transfer of foreign genes into calli takes place by Agrobacterium-va.Q6M.td transformation, particle bombardment, electroporation of protoplasts, or by viral vectors (Fischer... 

Dictyostelium discoideum cells can be transformed with plasmid DNA by convenient methods. The first attempts at this, using the calcium phosphate method, were reported in the early 1980s [17, 18]. Protocols were subsequently optimized by Firtel and coworkers [19, 20] and modified in many laboratories (e.g. Ref [21]). Up to 2000 transformants can be recovered from 10 D. discoideum cells [20], with electroporation protocols having been reported [22, 23]. It has also been noted that the choice of transformation method can strongly influence the yield of heterolo-gously expressed proteins [24]. Hence, both calcium co-precipitation and electroporation should be compared to generate optimized expression strains for the production of biopharmaceutical proteins in D. discoideum. 

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