Electrophoretic mobility schematic representation

Figure 1 Schematic representation of the relationship between the logarithmic normalized electrophoretic mobility (j l/j l0) and the molecular size (L). Rg, radius of gyration. (Reproduced with permission from Ref. 161.)...

Figure 11. Schematic representation of the electrophoretic mobility (A) measurement showing the major components. In an applied electric field, emulsion droplets move according to their surface charge. These charges can electrostatically stabilize an emulsion system by preventing the droplets from coming into contact and coalescing. The motion of the droplets is visually observed, and the electrophoretic mobilities of a number of particles are measured to determine zeta potential. The sedimentation potential (B) is also illustrated.

Figure 9.19. The diffuse double layer, (a) Diffuseness results from thermal motion in solution, (b) Schematic representation of ion binding on an oxide surface on the basis of the surface complexation model, s is the specific surface area (m kg ). Braces refer to concentrations in mol kg . (c) The electric surface potential, falls off (simplified model) with distance from the surface. The decrease with distance is exponential when l/ < 25 mV. At a distance k the potential has dropped by a factor of 1/c. This distance can be used as a measure of the extension (thickness) of l e double layer (see equation 40c). At the plane of shear (moving particle) a zeta potential can be established with the help of electrophoretic mobility measurements, (d) Variation of charge distribution (concentration of positive and negative ions) with distance from the surface (Z is the charge of the ion), (e) The net excess charge.

Figure 9. Schematic representation of electrophoretic mobility shift assay used to identify protein-DNA binding interactions.

The schematic representation of a CE apparatus is shown in Fig. 1. The mechanism of separation of water pollutants in CE is based on the electro-osmotic flow (EOF) and electrophoretic mobilities of the pollutants. The EOF propels all pollutants (cationic, neutral, and anionic) toward the detector and, ultimately, separation occurs due to the differences in the electrophoretic migration of the individual pollutants. Under the CE conditions, the migration of the pollutant is controlled by the sum of the intrinsic electrophoretic mobility (//ep) and the electro-osmotic mobility (/r o), due to the action of EOF. The observed mobility (Mobs)of the pollutants is related to and p p by the following equation ... 

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