Electrophoresis pore limit

Fawcett, JS Sullivan, SV Chrambach, A, Toward a Steady-State Pore Limit Electrophoresis Dimension for Native Proteins in Two-Dimensional Polyacrylamide Gel Electrophoresis, Electrophoresis 10, 182, 1989. 

CZE is a high resolution method provided that the sample is concentrated (>1 mg/ml) so that it can be loaded in a narrow zone. The buffer and pH for separation can be freely chosen, but buffer concentration should not exceed O.OIM to minimize Joule heating. In a homogeneous gel, separation occurs on the basis of both charge and size. In contrast, in a gradient polyacrylamide gel migration rates decrease until each protein species reaches its pore limit (15-17). This technique is termed "pore limit electrophoresis" and separates proteins on the basis of size. 

---