Electrophoresis general principles

Ref [i] AltriaKD (1996) General guidelines to the operation of capillary electrophoresis methods and instrumentation. In Altria KD (ed) Capillary electrophoresis guidebook principles, operation, and applications. Methods in molecular biology, vol. 52. Humana Press, Totowa... 

All types of electrophoresis are governed by the single set of general principles illustrated by Equation 4-1 ... 

Consider first the simple case an isolated, charged ion moving in a uniform electric field, as illustrated in Fig. 1. While this is an overly simplistic picture of electrophoretic motion in solution, it serves to illustrate the general principle. We will consider a more realistic model of electrophoresis in free solution in the following section. 

Further readinp Ritchie, R.F. and Smith, R. (1976). Im-munofixation. I. General principles and application to agarose gel electrophoresis. Clin, Chem., 22, 497... 

Capillary Electrophoresis. Capillary electrophoresis (ce) is an analytical technique that can achieve rapid high resolution separation of water-soluble components present in small sample volumes. The separations are generally based on the principle of electrically driven ions in solution. Selectivity can be varied by the alteration of pH, ionic strength, electrolyte composition, or by incorporation of additives. Typical examples of additives include organic solvents, surfactants (qv), and complexation agents (see Chelating agents). 

Our results prove that MChA also exists in electrical transport on a molecular scale, which implies that electrochemistry and electrophoresis in a magnetic field can in principle be asymmetric. Such experiments have been performed, but an initial positive result [39] could not be confirmed [40]. The photochemical results above show that MChA in general leads to quite small ee, even in very high magnetic fields, so the electrochemical experiments should be repeated with a carefully chosen model system, and with a very high sensitivity for the resulting ee. 

In general, partition of components from a solution at a solid surface provides the principle that is most often exploited (adsorption is used only very rarely), but for amino acids and peptides, which can exist in charged forms in aqueous solutions, ion-exchange and electrophoresis separation are also available. Separation on the basis of molecular size is also used. 

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