Electrophoresis and Isoelectric Focusing

Rodbard, D Chrambach, A, In Electrophoresis and Isoelectric Focusing in Polyacrylamide Gels Allen, RC Mauer, HR, eds. Walter E Gruyter New York, 1974 28. 

Hb-A2 can also be quantitated by electrophoresis. The most accurate procedures are starch block electrophoresis, cellulose acetate electrophoresis, and isoelectric focusing. 

Zhu, M., Hansen, D.L., Burd, S., and Gannon, F., Factors affecting free zone electrophoresis and isoelectric focusing in capillary electrophoresis, /. Ckromatogr., 480, 311, 1989. 

Hjerten, S., Elenbring, K., Kilar, F., Liao, J.-L, Chen, A. J. C., Siebert, C. J., and Zhu, M.-D., Carrier-free zone electrophoresis, displacement electrophoresis and isoelectric focusing in a high-performance electrophoresis apparatus, /. Chromatogr., 403, 47, 1987. 

Breci, L., Hattrup, E., Keeler, M., Letarte, J., Johnson, R., Haynes, PA. (2005). Comprehensive proteomics in yeast using chromatographic fractionation, gas phase fractionation, protein gel electrophoresis, and isoelectric focusing. Proteomics 5, 2018-2028. 

CaM was purified from porcine brain. The purity of proteins was examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and isoelectric focusing. CaM and PDE were cross-linked with l-ethyl-3(3-dimethylamino-propyl) carbodiimide (EDC) or N-succinimidyl 3-(2-pyridyldithio)propionate (SPDP) in a buffer solution of 0.1 M HEPES (pH 7.1) in the presence of 1 mM CaCl2. After a buffer solution containing 2 mM EGTA added into the reaction solution, the CaM-PDE hybrid was separated from other ingredients by gel chromatography on a Sepharose CL-6B solumn. 

L. Breci, E. Hattrup, M. Keeler, J. Letarte, R. Johnson, and P. A. Haynes. Comprehensive Proteomics in Yeast Using Chromatographic Fractionation, Gas Phase Fractionation, Protein Gel Electrophoresis, and Isoelectric Focusing. Proteomics, 5(2005) 2018-2028. 

Allen RC, Saravis CA, Maurer HR (1984) Gel electrophoresis and isoelectric focusing of proteins. Selected techniques. W. de Gruyter, Berlin Chrambach A, Dunn MJ, Radola BJ (eds.) (1987 foil.) Advances in electrophoresis, vol. 1 foil., VCH, Weinheim Hames BD, RickwoodD (eds.) (1990) Gel electrophoresis of proteins a practical approach, 2nd ed., Oxford University Press, New York Deyl Z, Chrambach A, Everaerts FM, PrusikZ (eds.) (1983) Electrophoresis. A survey of techniques and applications. J Chromatogr Library, vol. 18B, Elsevier, Amsterdam... 

Electrophoresis separates proteins on the basis of mass or charge. SDS gel electrophoresis and isoelectric focusing can be used separately or in combination for higher resolution. 

Electrophoresis and Isoelectric Focusing 141 Free induction decay... 

Fully equipped protein purification laboratories should also have preparative electrophoresis and isoelectric focusing apparatuses for rare occasions when other techniques fail to give sufficient separation. 

Miralles, B., Krause, I., Ramos, M., and Amigo, L. (2006). Comparison of capillary electrophoresis and isoelectric focusing for analysis of casein/caseinate addition in processed cheeses, hit. Dairy ]. 16,1448-1453. 

Other equally important methods are the electrophoretic techniques, such as polyacrylamide gel electrophoresis, isoelectric focusing, Western blots, combined electrophoresis, and isoelectric focusing (two-dimensional electrophoresis). Bioactivity methods are other key methods used in biotechnology product development. These include in vivo whole animal bioassay, cell culture bioassay, immunoassay, and biochemical assay. Many references and several textbooks are available in many industrial and academic libraries to provide additional and up-to-date information. 

Duley, J.A. Harris, 0. and Holmes, R.S. Analysis of human alcohol- and aldehyde-metabolizing isozymes by electrophoresis and isoelectric focusing. Alcohol Clin Exp Res 9 263-271, 1985. 

M.-D. "Carrier-free zone electrophoresis, displacement electrophoresis and isoelectric focusing in a high-performance electrophoresis apparatus" /. Chromatography. 1987, 403, pp 47-61. 

Courtesy - R.C. Allen, C.A. Saravis, H.R. Maurer, Gel Electrophoresis and Isoelectric Focusing of Proteins, de Gruyter, Berlin, Ger 1984)... 

Allen, R. C., Saravis, C. A., Maurer, H. R., "Gel Electrophoresis and Isoelectric Focusing of Proteins Selected Techniques", Walter de Gruyter, Berlin, 1984. 

Rodbard, D. Chrambach, A. In "Electrophoresis and Isoelectric Focusing in Polyacrylamide Gels" Allen, R.C. Maurer, H.R. 

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