Electronically transduced enzyme monolayers

ELECTRONICALLY TRANSDUCED PHOTOCHEMICAL SWITCHING OF ENZYME MONOLAYERS... 

Electronically Transduced Photochemical Switching of Enzyme Monolayers 227... 

Biosensors based on biorecognition events occurring in monolayer or thin-fihn assemblies on electronic transducers represent an important recent advance in bioelectronics [15]. The nano-architecture of the sensing interface in a mono-layer, multilayer, or thin-fihn structures precludes diffusion barriers, and hence the rapid response-times of the sensing devices are achieved. Enzyme-electrodes [16,17], immunosensors [18,19], or DNA sensors [13] were developed by tailoring nanoscale sensing interfaces on the transducer. We will specifically address the development of DNA sensors in monolayer and thin-film confignrations. 

Figure 3-5. (A) Assembly of reconstituted glucose oxidase on a PQQ-FAD monolayer linked to an Au-electrode. (H i Faradaic impedance spectra of the modified electrode at time intervals of reconstitution, (a) 0.1 h, (b) 0.25 h. (c) 0.5 h. (d) 1 h. (e) 2 h, (f) 4 h. Inset Interfacial electron transfer resistance of the modified electrode at time-intervals of reconstitution. (C) Cyclic voltammograms corresponding to the bioelectrocatalyzed oxidation of glucose, 80 mM, by the enzyme-functionalized electrode at time-intervals of reconstitution (a) 0 h, (b) 0.1 h, (c) 0.25 h, (d) 0.5 h, (e) 1 h, (f) 2 h, (g) 4 h. Inset Electrocatalytic currents transduced by the enzyme-modified electrode at time-intervals of reconstitution. Reproduced with permission from ref. 32. Copyright 2002 American Chemical Society.

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