Effluents, viii

TaM VIII. VACUUM RLTRATION CHARACTERISTICS OF UNCONOmONED DIGESTER EFFLUENT. RUN 7 TEST TEMPERATURE 26 C... 

M. Jonsson, E. Pettersson, and H. Rilbe, to be published. Isoelectric Fractional tion. Analysis, and Characterization of Ampholytes in Natural pH Gradients, VIII. Continuous Recording of pH and Light Absorbance of Column Effluent after Isoelectric Focusing. 

Fig. VIII-6. Chromatographic separation and identification of peptides in a thermolysin digest of TNP-clupeine YI. The digestion mixture (from 2.27 p,moles of TNP-clupeine YI) was applied to a column (0.9 cm x 150 cm) of CM-cellulose. Peptides were first eluted with the starting buffer (0.1 M acetate buffer, pH 6.0 tubes 1—80), then an exponential gradient elution was applied (81—514). The mixing chamber contained 1000 ml of starting buffer and to the reservoir 0.1 M acetate buffer (pH 6.5) containing 1.0 M NaCl was added. The peptides remaining on the column were eluted with 0.1 M HCl. The flow rate of the effluent was 3.89 ml/tube/18 min. The effluent was analyzed by Sakaguchi colorimetry and spectrophotometry at 340 mp. The color yield (Sakaguchi reaction) was 101 % (tubes 1 to 555) [from Ando and Suzuki, 1967 Suzuki and Ando 1972 (2)]...

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