Electrophoretic mobility effective

FIG. 28 Effective electrophoretic mobility in the case of hindered diffusion. (Reproduced with permission from Ref. 399.)... 

Ta = absolute temperature u = electrophoretic mobility Meff = effective electrophoretic mobility... 

Electrokinetic (also called electromigration) injection is performed by placing the inlet of the capillary and an electrode in the sample vial. Following this a voltage is applied during a defined period of time. The sample constituents are actively carried into the capillary, and when present, the EOF also passively carries them into the capillary. For this reason, neutral compounds are also injected. The active migration is due to the effective electrophoretic mobilities of the constituents. The amount (B), in units of concentration injected into the capillary is expressed by [2,38]... 

Figure 23 Fit of Effective Electrophoretic Mobilities, versus Q (Calculated According to the Relationship t,= q/MIm) for Peptides Separated Using a 50 mM Sodium Phosphate Buffer pH 3.0 324 ab...

Isotachophoresis (ITP) offers cm alternative to these chromatographic methods for the separation and chemical characterization of high-moleculeur-weight synthetic polymers containing an lonlzed)le functional group. The separations occur because of differences in the effective electrophoretic mobilities of the macromolecules. 

Figure 12. Dependence of effective electrophoretic mobility on poly (acryleunide-co-acrylate) composition.

Figure 8.1. Influence of buffer pH on the effective electrophoretic mobility of a monovalent weak acid in capillary electrophoresis. The separations show the relative position of the anion (A) and a neutral compound (M), used as an electroosmotic flow marker.

Relationships used to calculate the effective electrophoretic mobility of weak acids and bases... 

A low concentration of an ionic surfactant in the capillary electrolyte can also be used to modify the migration behavior of sample ions. A cationic surfactant such as cetyltrimethylammonium chloride (CTAC) is often used for anion separations, and SDS is often used when cations are to be separated. Kenata et al. [11] found that CTAC decreased the effective electrophoretic mobilities of several inorganic anions as the result of two processes ... 

A plot of the redprocal of the effective electrophoretic mobility for iodide against surfactant concentration gave two linear segments. The slope of the segment above the CMC was greater because of partitioning of iodide into the micelle. 

The association constants, K s, were measured by the method of Keneta et al. [11]. The reciprocal of measured, or effective, electrophoretic mobility of the analyte anion (Uef) is plotted against the concentration (C) of R4N in the capillary... 

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