Effect of Organic Modifier on Enantiomeric Separations

These data were generated with a 250 x 4.6 mm teicoplaiiiii column. Tlie mobile phase consisted of alcohol water (60/40, v/v), and the flow rate was 1 mL min at ambient temperature (23 °C). 

Flow rate was 0.5 mL min due to the viscosity of the propanol water mobile phase that produced a high backpressure. 

2 Method Development and Optimization of Enantiomeric Separations Using. .. 

This observation is important in the study of the chiral recognition mechanism in this system. This may be a practical matter when determining the trace amount of one enantiomer in the presence of its dominant antipode. The smaller peak is always desired to be eluted first for best quantitation. 

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