EcoRI

Econo Foam Economic evaluation EcoO EcoRl EcoRI Ecostatin... 

FIGURE 4.30 Calibration curves for double-stranded DNA fragments on TSK-GEL SW and TSK-GEL PW columns. Column TSK-GEL SW or TSK-GEL PW, two 7.5 mm X 60 cm columns in series. Sample 22 fragments from Hoelll-cleaved pBR322 DNA and 6 fragments from EcoRI-cleaved pBR322 DNA. Elution 0.1 M NaCI in 0.1 M phosphate buffer, pH 7.0, plus I mM EDTA Flow rate 1.0 ml/min. Detection UV at 260 nm. 

Blasiak J, Kowalik J. 1998. Interaction between organophosphoms compounds and DNA assayed by the restriction endonuclease EcoRI. Acta Univ Lodz Folia Biochim Biophys 13 31-67. 

Restriction enzymes are named after the bacterium from which they are isolated. For example, EcoRI is from Escherichia coli, and BamEII is from Bacillus amyloliquefaciens (Table 40-2). The first three letters in the restriction enzyme name consist of the first letter of the genus (E) and the first two letters of the species (co). These may be followed by a strain designation (R) and a roman numeral (I) to indicate the order of discov-ery (eg, EcoRI, EcoRIE). Each enzyme recognizes and cleaves a specific double-stranded DNA sequence that is 4—7 bp long. These DNA cuts result in blunt ends (eg,... 

EcoRI 4 GAATTC CHAAG T Escherichia coli RY13... 

Cutting with restriction endonucleases is very useful for moving specific pieces of DNA around from place to place. It s also a useful way to name pieces of DNA. For example, a piece of DNA that is cut from a bigger piece of DNA is often named by size and given a surname that corresponds to the two restriction enzymes that did the cutting—the 0.3-kb EcoRI-BamHI fragment. Restriction enzymes themselves are named for the bacterial strains from which they were initially isolated. 

The localization of bNOS to the human genome was accomplished by Kishimoto and coworkers70. These investigators used a rat cerebellar cDNA to obtain a human cDNA from Clontech. This cDNA was hybridized to Southern blots containing DNA from a battery of human-rodent somatic cell DNA. Since the blots had been shown to be selective, the authors showed that the cDNA hybridized to chromosome 12. By using restriction nucleases EcoRI and Hind in the assignment was made to 12 ql4-qter. One or two copies were indicated in vivo but reducing the hybrid conditions showed more bands. It is necessary to conduct further studies to see whether the other cDNA are derived from this or another clone. 

EcoRI Escherichia coli RY13 G AATTC Sticky... 

This Is the DNA sequence recognized by the restriction endonuclease EcoRI. Rgure 1-6-1. EcoRI Recognition Sequence... 

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