Early Work by West and Associates

West and Nichols in 1928 (120), starting with a commercial extract (fraction G of Cohn), and following the methods of further purification as developed by Cohn—i.e., use of basic lead acetate, phosphotungstic acid and silver, obtained a product the analyses of which they reported as  

12-14 per cent 20 per cent of total N 40 per cent of total N None 

They also reported that their material had the following qualitative properties  

In 1930, West and Howe (116,117) reported the preparation of a product which on intravenous injection of 680 mgm. caused a reticulocyte rise from 3 per cent to 40 per cent, with a red cell rise from 1.4 to 2.3 millions per cubic mm. This material, obtained as an amorphous precipitate, was acid to methyl red and showed a levorotation of about —15 degrees. 

It yielded amorphous barium and green copper salts which were soluble in HjO and precipitable by alcohol. Other properties which characterized the material were  

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