DryLab resolution map

The following example illustrates the value of an initial negative result The sample was a pure drug substance spiked with five possible impurihes the goal was separation of aU peaks. The effects of both gradient time and temperature on separation were first determined by means of just four experimental mns 10-90% MeCN/water in 15 min and 90 min at 25 °C and 45 °C. The DryLab resolution map and best resulting separahon are shown in Fig. 13, indicating no possibility of a satisfactory separation in this way. 

The DryLab resolution map (Fig. 15) shows that a promising result can be achieved within these experimental boundaries, with maximum resolution of the two impurity peaks (the first peaks in the chromatogram) at a temperature of 35 °C. 

Initial DryLab resolution map and predicted gradient separation. 

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