Drug candidates screening

Automated online SPE systems have been applied to various phases of drug discovery. McLoughlin et al. (1997) utilized the Prospekt system in pharmacokinetic animal studies for rapid drug candidate screening. Up to 10 compounds were simultaneously monitored. The lower limits of detection were... 

Zhang, H., Pan, Y Affinity ultrafiltration of DNA topoisomerases-targeted compounds determined with HPLC/ESI-MS for drug candidate screening. J. Zhqiang Univ. Sci. 2004, 5, 900-905. 

Fortunately, robot-based drug candidate screening systems represent a success story for time-to-market and labour- and time-saving reasons, these systems are making a comeback in this specific pharmaceutical field. 

The analysis of a molecule with one site of action is a good indicator for the LD50, such as in phenacyl alcohol derivatives. Steroids, however, are complex molecules, and their metabolites contribute to the experimentally measured LD50 values. If the toxicity can be related to superoxide, the measurement of chemiluminescence intensity can be used for a drug candidate screening. Further studies of the substituent effect of steroids are required before LD50 values can be estimated from computational chemical calculations. 

Case Study 1 Drug Candidate-Induced Direct Acinar Cell Toxicity In Vivo with Confirmation of Toxicity and Drug Candidate Screening In Vitro... 

The drug discovery process can be divided into four subsets acquisition of chemical drug candidates, pharmacodynamic testing of large numbers of compounds (screening), and the optimization of pharmacokinetic and pharmaceutical properties. 

Uetrecht J. Screening for the potential of a drug candidate to cause idiosyncratic drug reactions. Drug Discov Today 2003 8 832-7. 

Predictions of no, or low, toxicity in a general drug screening approach should be used to indicate a possible absence of toxicity in potential drug candidates. Inevitably this would require further toxicological assessment of potential drugs to ensure safety. 

High-throughput screening (HTS) is today the most commonplace method for identifying lead compounds that can be subsequently optimized to generate drug candidates. 

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