Drug biotransformation perfused organs

The introduction of in vitro techniques (microsomal preparations, tissue cultures, perfused organ systems, or purified enzymes) was meant to overcome many of those problems unique to whole animals systems. Because liver is the major site of chemical and drug biotransformation, it is the tissue of choice for metabolism and toxicity studies, the majority of which are conducted using microsomal preparations [16]. While there is considerable impetus to perform metabolism studies using this technique, they may be complicated by several commonly encountered practical problems, including [13] ... 

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